Purpose

This laboratory study is collecting and storing tissue, blood, and bone marrow samples from young patients with cancer. Collecting and storing samples of tissue, blood, and bone marrow from patients with cancer to study in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer.

Conditions

Eligibility

Eligible Ages
Under 21 Years
Eligible Genders
All
Accepts Healthy Volunteers
No

Criteria


Inclusion Criteria:

- All malignant tissues from childhood cancers allowed including the following:

- Brain tumors (all types)

- Tissue should be submitted to CNS Committee Resource labs to be forwarded
for this study, unless instructed otherwise on the COG web site

- Ewing family of tumors

- Rhabdomyosarcomas

- Other soft tissue sarcomas

- Osteogenic sarcomas

- Rhabdoid tumors

- Neuroblastomas

- Viable material for cell culture for neuroblastoma is collected via
COG-ANBL00B1 and should not be submitted via this study unless the patient
cannot be enrolled on COG-ANBL00B1*

- Retinoblastomas

- Anaplastic Wilms tumor

- Germ cell tumors

- Leukemias/lymphomas

- Acute myeloid leukemia (AML)

- Blood samples and bone marrow samples from patients at second relapse
and beyond may be submitted for this study

- Bone marrow samples at diagnosis or first relapse must be submitted to
an AML resource lab and will be forwarded for this study at the
discretion of the AML Committee

- Acute lymphoblastic leukemia (ALL)

- Blood samples may be submitted directly to this study

- Bone marrow samples must be submitted to an ALL resource lab and will
be forwarded for this study at the discretion of the ALL Committee

- Enrolled on a COG therapeutic, biology, or tissue banking protocol that allows
collection of tissue for research and submission to a COG-designated resource
laboratory

- Participation in this protocol is not permitted until after tissue requirements
for any active COG disease-specific therapeutic, biology, or banking protocols
have been satisfied

- Material may only be submitted for this protocol if tissue is available in excess
of that required for satisfying active disease-specific therapeutic and
biological protocols

- Patients with diagnosis pending are eligible

Study Design

Phase
Study Type
Observational
Observational Model
Case-Only
Time Perspective
Cross-Sectional

Arm Groups

ArmDescriptionAssigned Intervention
Ancillary-Correlative (tissue sample collection) Leftover tissue from diagnostic procedures and/or surgery is cryopreserved and banked. Blood and/or bone marrow are also collected and banked. Cell lines are established and characterized via reverse-transcriptase polymerase chain reaction and/or flow cytometry for biomarkers and by DNA fingerprinting. Markers to be identified may include the following: NEUROBLASTOMA: tyrosine hydroxylase, protein gene product (PGP) 9.5, GD2, HLA class I, and HSAN 1.2 antigens EWING FAMILY OF TUMORS: EWS-FLI1, EWS-ERG, and PGP 9.5 RETINOBLASTOMA: interphotoreceptor retinoid-binding protein ACUTE LYMPHOBLASTIC LEUKEMIA: immunophenotype ALVEOLOR RHADOMYOSARCOMA: PAX3-FKHR, PAX7-FKHR, and MyoD1 ALL CELL TYPES: telomerase expression including hTR and hTERTMutations of TP53 gene are detected by flow cytometry and/or immunocytochemistry
  • Other: Cytology Specimen Collection Procedure
    Correlative studies
    Other names:
    • Cytologic Sampling
  • Other: Laboratory Biomarker Analysis
    Correlative studies

Recruiting Locations

Montefiore Medical Center - Moses Campus
Bronx, New York 10467
Contact:
Site Public Contact
718-379-6866
aaraiza@montefiore.org

More Details

Status
Recruiting
Sponsor
Children's Oncology Group

Study Contact

Detailed Description

PRIMARY OBJECTIVES: I. Establish and bank cell lines and/or xenografts from pediatric patients with cancer. II. Establish continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer. III. Establish transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro. IV. Create a bank of cell lines and generate sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols. V. Characterize cell lines from childhood cancers with respect to DNA short tandem repeat molecular profile as a "fingerprint" of original cell line identity. VI. Characterize cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts. VII. Characterize cell lines for mycoplasma contamination. VIII. Characterize cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance. OUTLINE: This is a multicenter study. Specimens are stratified according to disease (acute lymphoblastic leukemia vs acute myeloid leukemia vs lymphoma vs osteogenic sarcoma vs Ewing family of tumors vs rhabdomyosarcoma vs primitive neuroectodermal tumor vs glioma vs astrocytoma vs rhabdoid tumors vs hepatoblastoma vs retinoblastoma vs Wilms tumor vs germ cell tumors vs other diagnoses). Leftover tissue from diagnostic procedures and/or surgery is cryopreserved and banked. Blood and/or bone marrow are also collected and banked. Cell lines are established and characterized via reverse-transcriptase polymerase chain reaction and/or flow cytometry for biomarkers and by DNA fingerprinting. Markers to be identified may include the following: NEUROBLASTOMA: tyrosine hydroxylase, protein gene product (PGP) 9.5, GD2, HLA class I, and HSAN 1.2 antigens EWING FAMILY OF TUMORS: EWS-FLI1, EWS-ERG, and PGP 9.5 RETINOBLASTOMA: interphotoreceptor retinoid-binding protein ACUTE LYMPHOBLASTIC LEUKEMIA: immunophenotype ALVEOLOR RHADOMYOSARCOMA: PAX3-FKHR, PAX7-FKHR, and MyoD1 ALL CELL TYPES: telomerase expression including hTR and hTERTMutations of TP53 gene are detected by flow cytometry and/or immunocytochemistry. No results of these tests are provided to the patient, the patient's physician, or the patient's medical records.

Notice

Study information shown on this site is derived from ClinicalTrials.gov (a public registry operated by the National Institutes of Health). The listing of studies provided is not certain to be all studies for which you might be eligible. Furthermore, study eligibility requirements can be difficult to understand and may change over time, so it is wise to speak with your medical care provider and individual research study teams when making decisions related to participation.